movat pentachrome  (StatLab Medical Products Inc)

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: Single-Cell and Spatial Transcriptomics Identified Fatty Acid–Binding Proteins Controlling Endothelial Glycolytic and Arterial Programming in Pulmonary Hypertension

doi: 10.1161/ATVBAHA.124.321173

Figure Lengend Snippet: FABP (fatty acid–binding protein) 4 and FABP5 deletions protected from Egln1-deficiency–induced pulmonary hypertension in mice. A , A diagram showing the strategy for generation of FABP4 and FABP5 double knockouts in Egln1 Tie2Cre mice (TKO). B , Representative Western blotting demonstrating diminished FABP4 and FABP5 protein expression in lung lysate isolated from TKO lungs compared with CKO lungs. C , Dramatic reduction in right ventricular (RV) systolic pressure (RVSP) was seen in TKO mice compared with CKO mice. Bars represent the mean. D , Marked inhibition of RV hypertrophy in TKO mice compared with CKO mice. E and F , Representative images from Russel-Movat pentachrome staining and quantification of wall thickness of pulmonary artery (PA) exhibited reduced thickness in the intima, media, and adventitia layers of tissue of TKO mice compared with CKO mice. Occlusive lesions were diminished in TKO mice compared with CKO mice. G , Exemplary images showed a reduction in muscularization of distal pulmonary vessels in TKO mice. Lung sections were subjected to immunostaining using anti–α-SMA (α-smooth muscle actin). H , Muscularization quantification was performed by tallying SMA-positive vessels across 40 fields (at ×20 magnification) within every lung section. The results were presented as the mean value along with the SD (mean ± SD) based on a sample size of 5 to 10 mice per group. ANOVA followed by Tukey post hoc analysis was used for statistical analysis ( B , C , D , F , and H ). A.U. indicates arbitrary unit; Br, bronchus; DAPI, 4',6-diamidino-2-phenylindole; RV(LV+S), RV and the left ventricle plus septum; V, vessel; and WT, wild-type.

Article Snippet: Lung sections were stained with a Russel-Movat pentachrome staining kit (catalog no. KTRMP, StatLab) according to the manufacturer’s protocol.

Techniques: Binding Assay, Western Blot, Expressing, Isolation, Inhibition, Staining, Immunostaining